With a 25g, the puncture left in my vial seems huge, so I moved to 30g. Either one should probably do the job, correct? There isn’t any particular advantage a 25g offers compared with a 30g, is there? It just comes down to what someone likes?
The variation in dimensions isn't as large as you'd expect, and the diameter of the "hole" is irrelevant since re-using a vial after extracting the liquid for filtration is something you should NOT do—doing so would defeat the whole point of filtering.
My bad, I didn't phrase that well.
When it comes to other peps, I've run 4-5mL through, but for someone only using tirz at 1-2mL, a 4mm filter ought to work just fine. As for T100, I honestly wouldn't have a clue what to do with it. At 5mg I'm nearly at maintenance, so it would spoil long before I managed to finish it. lol
Yes, I do run it through a filter into a fresh sterile vial, but the puncture it makes in the stopper seems larger. Perhaps the vials I'm using aren't the correct ones.
When I was reconstituting, my 1 inch 30 gauge kept bending, so I switched to a larger size.
Right — you start by reconstituting it. Once that's done, pull the solution up into a syringe. Next, remove the needle, attach the filter, mount a fresh needle, and inject into a new vial.
Absolutely. For the BAC, I use a 23g 1" to inject it, then a 23g 1.5" to pull it out, and a separate 23g 1" to push it through the filter into the fresh vial. Boxes of 100 of these 3ml and 5ml luer lock syringes and needles don't cost much on Amazon. Going through that many doesn't bother me.
It seems like the needle you use to draw the contaminated solution is the same one you then use to push the filtered liquid into a sterile vial — which defeats the purpose of filtering all over again.
This is the first time I've come across anyone putting a filter on the venting needle. I assume it's to filter the incoming air? That's an interesting concept. There was a whole conversation we had regarding non-sterile air entering the vial. Still, I'm curious whether it actually does anything when it's just air. Is this a common practice?
I picked up this habit from a PeptideTest filtering clip. Plenty of folks roll their eyes and claim it's just a way to push more filter sales, but to me the logic held up, so I keep doing it. If my end goal were vials, then yeah, air has to be introduced eventually or you'd be pulling against a vacuum just to draw liquid out, so in that case I probably wouldn't bother. But since I'm pushing into cartridges, the moment the peptide is in, the first step is clearing out every bit of air and none ever gets added afterward, so it strikes me as a sensible thing to do for that setup.
