Reconstitution question

[DJ _Yus]

Noob question,

I mixed my first vial. While drawing the BAC water, the syringe showed only about 2.6 ml of BAC. (I even opened the entire vial to try to get all of it out) Then I combined this with my Reta 60mg.

If my starting dose is 2mg per week, then based on the calculators my dose should be 9 units.

Is that math right?

Also, how often does BAC come underfilled?

Pics for reference

 

[skaylife]

DJ _Yus said:

Noob question,

I mixed my first vial. While drawing the BAC water, the syringe showed only about 2.6 ml of BAC. (I even opened the entire vial to try to get all of it out) Then I combined this with my Reta 60mg.

If my starting dose is 2mg per week, then based on the calculators my dose should be 9 units.

Is that math right?

Also, how often does BAC come underfilled?

Pics for reference





View attachment 11448

View attachment 11449

The math checks out, but the way you went about it does not. Vials should never be opened. Once a vial is open, air can contaminate it. That matters far more than using gloves.

 

[aRareUnicorn]

the math works, but… a 60mg vial is a lot for a starter, even titrating up will take forever to get through… and the contamination risk goes up…

 

[WholesomeApril]

Two issues here. First, removing the whole lid from the Reta wasn’t necessary. By doing that, you’ve likely introduced contaminants when the rubber stopper should have stayed in place—you simply insert your insulin syringe through the center of the stopper to pull your dose.

Second, if you’re taking 2 mg per week, your vial will remain in the fridge far beyond the recommended time. Personally, I wouldn’t be at ease using Reta that’s been stored for more than about 6 to 8 weeks.

 

[sheilarae74]

DJ _Yus said:

Noob question,

I mixed my first vial. While drawing the BAC water, the syringe showed only about 2.6 ml of BAC. (I even opened the entire vial to try to get all of it out) Then I combined this with my Reta 60mg.

If my starting dose is 2mg per week, then based on the calculators my dose should be 9 units.

Is that math right?

Also, how often does BAC come underfilled?

Pics for reference





View attachment 11448

View attachment 11449

For someone just beginning, 60 mg is quite a high amount. I started with a 20 mg vial and it covered 9 weeks for me. Because you've already opened the vial, using it for that length of time is definitely not something I'd do. At this point, I doubt filtering would change anything.

If you want the fundamentals of reconstitution, Peptide Critic is a solid resource

View: https://youtu.be/geNpx5sJH3k?si=JQIYnmFFZHqWcJvT

 

[lessthanhalf]

You're only supposed to take the plastic top off the rubber stopper, not the whole rubber stopper. After that, you'll see a metal ring around the vial's edge, and in the center there's the spot where needles go through the rubber stopper to add bac water or draw doses. Before a needle touches it, wipe the rubber stopper with an alcohol wipe.

From what I can see, you've also removed the rubber stopper from the reta vial. Once that's done, there's zero chance it stays sterile for weeks, so you should probably toss it and start over, though the first dose should be ok. Bacteria or fungi will grow over that time.

If 1 vial doesn't have enough bac, use 2 to reach a nice round number like 2ml or 3 ml so calculations are simpler. At 3ml per 60mg reta, that's 1ml per 20mg or 0.1ml per 2mg. At such low volumes, being precise about doses is going to be hard, probably doesn't matter much, but with bubbles in the way etc doses are going to be plus or minus 10%

 

[DJ _Yus]

lessthanhalf said:

You're only supposed to take the plastic top off the rubber stopper, not the whole rubber stopper. After that, you'll see a metal ring around the vial's edge, and in the center there's the spot where needles go through the rubber stopper to add bac water or draw doses. Before a needle touches it, wipe the rubber stopper with an alcohol wipe.

From what I can see, you've also removed the rubber stopper from the reta vial. Once that's done, there's zero chance it stays sterile for weeks, so you should probably toss it and start over, though the first dose should be ok. Bacteria or fungi will grow over that time.

If 1 vial doesn't have enough bac, use 2 to reach a nice round number like 2ml or 3 ml so calculations are simpler. At 3ml per 60mg reta, that's 1ml per 20mg or 0.1ml per 2mg. At such low volumes, being precise about doses is going to be hard, probably doesn't matter much, but with bubbles in the way etc doses are going to be plus or minus 10%

If there's no cloudiness or growth, could I keep using this Reta for about a couple weeks — say 4 doses — until a new vial arrives?

 

[anhydrous]

DJ _Yus said:

lessthanhalf said:

You're only supposed to take the plastic top off the rubber stopper, not the whole rubber stopper. After that, you'll see a metal ring around the vial's edge, and in the center there's the spot where needles go through the rubber stopper to add bac water or draw doses. Before a needle touches it, wipe the rubber stopper with an alcohol wipe.

From what I can see, you've also removed the rubber stopper from the reta vial. Once that's done, there's zero chance it stays sterile for weeks, so you should probably toss it and start over, though the first dose should be ok. Bacteria or fungi will grow over that time.

If 1 vial doesn't have enough bac, use 2 to reach a nice round number like 2ml or 3 ml so calculations are simpler. At 3ml per 60mg reta, that's 1ml per 20mg or 0.1ml per 2mg. At such low volumes, being precise about doses is going to be hard, probably doesn't matter much, but with bubbles in the way etc doses are going to be plus or minus 10%

If there's no cloudiness or growth, could I keep using this Reta for about a couple weeks — say 4 doses — until a new vial arrives?

Because it has come into contact with ambient air, the whole solution has to be passed through a PES 0.22um syringe filter and deposited into a different sterile vial. After that step, I would go ahead and use it, acting as though this situation never occurred.

 

[Flash-BCR]

I've got Reta kits at 20mg...but I picked up some 10mg Reta to get around this issue with the starting dose...

 

[lessthanhalf]

DJ _Yus said:

lessthanhalf said:

You're only supposed to take the plastic top off the rubber stopper, not the whole rubber stopper. After that, you'll see a metal ring around the vial's edge, and in the center there's the spot where needles go through the rubber stopper to add bac water or draw doses. Before a needle touches it, wipe the rubber stopper with an alcohol wipe.

From what I can see, you've also removed the rubber stopper from the reta vial. Once that's done, there's zero chance it stays sterile for weeks, so you should probably toss it and start over, though the first dose should be ok. Bacteria or fungi will grow over that time.

If 1 vial doesn't have enough bac, use 2 to reach a nice round number like 2ml or 3 ml so calculations are simpler. At 3ml per 60mg reta, that's 1ml per 20mg or 0.1ml per 2mg. At such low volumes, being precise about doses is going to be hard, probably doesn't matter much, but with bubbles in the way etc doses are going to be plus or minus 10%

If there's no cloudiness or growth, could I keep using this Reta for about a couple weeks — say 4 doses — until a new vial arrives?

Ultimately, the decision rests with you. If you inject contaminants beneath the skin into the subcutaneous layer, there will be some level of risk. In most cases, your immune system eliminates them and nothing comes of it. The next most probable outcome is a minor localized abscess that may require antibiotics. A more serious scenario arises if the contaminants are virulent: an invasive soft tissue infection that spreads, which would certainly call for antibiotics and could necessitate hospital-administered IV antibiotics. The most severe possibility is the infection entering the bloodstream, which without antibiotics could be fatal. None of these outcomes is particularly likely, and no one can estimate the odds. My view is that the first dose is acceptable, since there was no opportunity for any contaminants to multiply even if some were introduced. Beyond that, however, even with bac water, contaminants could proliferate, and the risk grows over time—likely not a risk worth accepting.

 

[Mochizuki0124]

Before I ever touched any peptides, I had already gone through tons of videos about injecting them, and particularly about how to reconstitute them.

I don't mean to be harsh, but right now I don't believe you're at a point where peptides make sense for you. My advice: treat this as a 60mg lesson, then get a new vial. Before you do anything with that next vial, study up on reconstitution AND on storage. Spending 60mg hurts a lot less than spending on medical care (and peptides already carry that risk). On top of that, 60mg is way too much for a first run with reta. I began at .5mg per week and it still took me a while to adjust.

 

[BNLFL]

aRareUnicorn said:

the math works, but… a 60mg vial is a lot for a starter, even titrating up will take forever to get through… and the contamination risk goes up…

That was among the initial details I picked up on. That works out to 30 doses.

 

[5byfive]

Time for some tough love. You've got to step away from the gray market. Everything you did wrong makes it obvious that you hadn't read or researched a single thing before diving into choices that can seriously put your health at risk. I hate to say it, but gray isn't meant for everybody, and this proves it isn't meant for you. Not everyone has the mindset, the skill to weigh risk, the patience to do reasesrch, or the judgment this requires. This might come across as harsh, but I'm doing my best to be kind while making sure you understand how serious this is. This just isn't for you.

 

[flapjacks]

Soooo, not to kill your vibe, but bacteria do exist. Perhaps you should look into things more thoroughly BEFORE injecting yourself with anything. I'm no professional, but keeping things sterile is essential for success.

 

[DJ _Yus]

anhydrous said:

DJ _Yus said:

lessthanhalf said:

You're only supposed to take the plastic top off the rubber stopper, not the whole rubber stopper. After that, you'll see a metal ring around the vial's edge, and in the center there's the spot where needles go through the rubber stopper to add bac water or draw doses. Before a needle touches it, wipe the rubber stopper with an alcohol wipe.

From what I can see, you've also removed the rubber stopper from the reta vial. Once that's done, there's zero chance it stays sterile for weeks, so you should probably toss it and start over, though the first dose should be ok. Bacteria or fungi will grow over that time.

If 1 vial doesn't have enough bac, use 2 to reach a nice round number like 2ml or 3 ml so calculations are simpler. At 3ml per 60mg reta, that's 1ml per 20mg or 0.1ml per 2mg. At such low volumes, being precise about doses is going to be hard, probably doesn't matter much, but with bubbles in the way etc doses are going to be plus or minus 10%

If there's no cloudiness or growth, could I keep using this Reta for about a couple weeks — say 4 doses — until a new vial arrives?

Because it has come into contact with ambient air, the whole solution has to be passed through a PES 0.22um syringe filter and deposited into a different sterile vial. After that step, I would go ahead and use it, acting as though this situation never occurred.

So if I pass it through a filter, is it still fine to use?

 

[anhydrous]

DJ _Yus said:

anhydrous said:

DJ _Yus said:

lessthanhalf said:

You're only supposed to take the plastic top off the rubber stopper, not the whole rubber stopper. After that, you'll see a metal ring around the vial's edge, and in the center there's the spot where needles go through the rubber stopper to add bac water or draw doses. Before a needle touches it, wipe the rubber stopper with an alcohol wipe.

From what I can see, you've also removed the rubber stopper from the reta vial. Once that's done, there's zero chance it stays sterile for weeks, so you should probably toss it and start over, though the first dose should be ok. Bacteria or fungi will grow over that time.

If 1 vial doesn't have enough bac, use 2 to reach a nice round number like 2ml or 3 ml so calculations are simpler. At 3ml per 60mg reta, that's 1ml per 20mg or 0.1ml per 2mg. At such low volumes, being precise about doses is going to be hard, probably doesn't matter much, but with bubbles in the way etc doses are going to be plus or minus 10%

If there's no cloudiness or growth, could I keep using this Reta for about a couple weeks — say 4 doses — until a new vial arrives?

Because it has come into contact with ambient air, the whole solution has to be passed through a PES 0.22um syringe filter and deposited into a different sterile vial. After that step, I would go ahead and use it, acting as though this situation never occurred.

So if I pass it through a filter, is it still fine to use?

Should bacteria, fungi, molds, and particulates be a worry, a PES 0.22um syringe filter is able to take those out of the mix.

 

[byefatlicia]

What made you take the stopper out of the Reta vial?

 

[Vriende]

DJ _Yus said:

anhydrous said:

DJ _Yus said:

lessthanhalf said:

You're only supposed to take the plastic top off the rubber stopper, not the whole rubber stopper. After that, you'll see a metal ring around the vial's edge, and in the center there's the spot where needles go through the rubber stopper to add bac water or draw doses. Before a needle touches it, wipe the rubber stopper with an alcohol wipe.

From what I can see, you've also removed the rubber stopper from the reta vial. Once that's done, there's zero chance it stays sterile for weeks, so you should probably toss it and start over, though the first dose should be ok. Bacteria or fungi will grow over that time.

If 1 vial doesn't have enough bac, use 2 to reach a nice round number like 2ml or 3 ml so calculations are simpler. At 3ml per 60mg reta, that's 1ml per 20mg or 0.1ml per 2mg. At such low volumes, being precise about doses is going to be hard, probably doesn't matter much, but with bubbles in the way etc doses are going to be plus or minus 10%

If there's no cloudiness or growth, could I keep using this Reta for about a couple weeks — say 4 doses — until a new vial arrives?

Because it has come into contact with ambient air, the whole solution has to be passed through a PES 0.22um syringe filter and deposited into a different sterile vial. After that step, I would go ahead and use it, acting as though this situation never occurred.

So if I pass it through a filter, is it still fine to use?

Nope!

Toss it in the trash.

Because you didn't understand what Mochizuki0124, 5byfive and flapjacks explained earlier, take several more weeks to read up before you even consider beginning again.

 

[deleted.user.27]

I’ve visited the sites that experienced people here have shared for medical supplies. My ignorance about peptide filtering might explain why I never searched for or spotted filters. If I revisit those sites, will they have filters available to buy? And can a filter be used multiple times, or is it strictly single-use?

 

[deleted.user.27]

Ignore this, I already came across the solution in a different thread on this site. I ought to have searched around before posting, that's on me.