lessthanhalf said:
The fact that you're feeling pronounced effects from doses below what I'd expect gives me some pause.
There's a very rough way to translate rodent doses into human ones: take 1/10th of the rodent mg/kg figure. Applying that, the 10mg/kg-100mg/kg range used in rodent research works out to a human equivalent of 1-10mg/kg, which for someone weighing 100kg comes to 100mg up to 1g per day. I won't claim this method is precise, but it generally lands you in the right ballpark, or at minimum the right order of magnitude.
Even so, running the numbers this way, the smallest dose used in mouse studies comes out to 1mg/kg/day, or 60 to 100mg/day in people - and all of that is based on oral administration. I went to the trouble of working this out because the animal data are interesting. Your reports, though, point to stronger effects at doses around 1/30th to 1/12th of the lowest figure I arrived at. Since heart rate is the simplest thing to measure, pushing it to 100 doesn't sound like a safe dose - though a placebo response is also possible, and that's exactly the trouble with n of 1 studies that aren't blinded. It also argues that dose-finding phase 1 trials genuinely ought to happen before anyone uses this in humans. Please don't take any of this as dosing advice; based on heart rate, it sounds like your doses are too high rather than too low. I've seen oral doses reported online ranging anywhere from 0.5mg to 200mg, and that spread is remarkably wide. Vendors sometimes intentionally sell drugs at low doses when they suspect there could be reasons to worry about adverse effects. The bulk of the animal work used oral dosing, and while bioavailability isn't especially high given how polar the molecule is, it isn't poor enough to account for a 12-100x shift between oral and parenteral routes.
If you do decide to go ahead, my suggestion would be to measure effects more systematically - particularly body temperature, blood pressure and heart rate, taken both before dosing for a baseline and afterward. And weight too. Any elevation in body temperature is a serious concern and means the dose is too high, and the same goes for a heart rate increase beyond 10 bpm or a rise in blood pressure. With enough measurements, hopefully non-drug influences like expectation or placebo would tend to fade and leave the results somewhat cleaner.
