Peptide pens, dosage and temperature changes

[DJ _Yus]

After some reading I'm still not clear about the best practice for when getting weekly pin, from my pen that sits on the fridge between doses.

Some people say, to let the reconstituted pep to come to room temperature, and then pin.

Some people say, to keep the pep out of the fridge as little as possible to prevent temperature fluctuations that will cause biodegradation faster. Meaning you should pin fast when taking the pep out of the fridge.

What do you guys think, and what practices do you follow?

 

[SeaGypsy]

I use my pens straight from the fridge then immediately back in they go. Warm /cool cycles cannot be good for the pep. If you feel you need to let it come to room temp do not use a pen, just pull your dose from the vial and put the vial back in the fridge.

 

[desinr-gal]

SeaGypsy said:


I use my pens straight from the fridge then immediately back in they go. Warm /cool cycles cannot be good for the pep. If you feel you need to let it come to room temp do not use a pen, just pull your dose from the vial and put the vial back in the fridge.

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Totally agree with this. The cold doesn't bother me at all, and mine last for months with no problem. Constant flux in temp change is said to weaken the peptides.

 

[Grogu]

SeaGypsy said:


I use my pens straight from the fridge then immediately back in they go. Warm /cool cycles cannot be good for the pep. If you feel you need to let it come to room temp do not use a pen, just pull your dose from the vial and put the vial back in the fridge.

Click to expand...

I agree with all of this, but just add that some peptides cause less pain at room temperature and in those cases what you suggest is the best approach.

The only "benefits" I can see of putting a weekly peptide in a pen is the fact that you can expel the extra air from the cartridge. I really like that for some reason. And also not puncturing a vial, pen needles are super sharp. I never thought that just one puncture of a vial stopper would dull a needle, but yup, it does. Otherwise, pens for weekly injections only speed things up by a fraction.

 

[desinr-gal]

Grogu said:


I agree with all of this, but just add that some peptides cause less pain at room temperature and in those cases what you suggest is the best approach.

The only "benefits" I can see of putting a weekly peptide in a pen is the fact that you can expel the extra air from the cartridge. I really like that for some reason. And also not puncturing a vial, pen needles are super sharp. I never thought that just one puncture of a vial stopper would dull a needle, but yup, it does. Otherwise, pens for weekly injections only speed things up by a fraction.

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It can save a lot of time and Plastic, if you are using the same vial for a longer period of time. My 30mg vials last for 30 pins @ 1mg per pin, every 4 days, that lasts 4 months.

pen needles are much better too.

 

[SeaGypsy]

Grogu said:


I agree with all of this, but just add that some peptides cause less pain at room temperature and in those cases what you suggest is the best approach.

The only "benefits" I can see of putting a weekly peptide in a pen is the fact that you can expel the extra air from the cartridge. I really like that for some reason. And also not puncturing a vial, pen needles are super sharp. I never thought that just one puncture of a vial stopper would dull a needle, but yup, it does. Otherwise, pens for weekly injections only speed things up by a fraction.

Click to expand...

Yes, that's one of the reasons I love the pen needles, always sharp and smooth. I rarely ever feel one during the stick.

 

[eidos]

You’ll get as many answers as there are people on this forum.



From a physical chemistry perspective:

• There is no change in the state of the water during reconstitution (from solid to liquid). Therefore, there is no physical stress on the solutes.
  
• The temperature is in Kelvin in the reaction equations (I’m keeping it simple , so going from 2°C to 25°C means going from 275K to 298K. That’s less than a 10% change in temperature.
  
• For GLP-1s, there are a significant number of hydrogen bonds and hydrophobic interactions, which means that at these temperatures, the change in energy (ΔG) remains below 10%.
  
• An increase in temperature increases solubility and will break up certain hydrophobic aggregates (don’t bother looking: you can’t see them with the naked eye; if you do see them, your solution is spoiled).
  
• Adsorption onto glass walls is minimal at typical reconstitution concentrations (on the order of 1 per thousand), so it is not a factor in our situations.

In general, avoid temperatures above 25°C. Significant degradation occurs at temperatures above 30°C (deamidations, accelerated oxidation).

There’s no need to bring it up to room temperature. You can stop at around 15°C (you can easily find cases with integrated thermometers). That will be enough to increase solubility and prevent thermal shock, which some white blood cells don’t like (some people are allergic to the cold...).

So, it's fine to warm it up, and while it's not mandatory, it helps a little.

 

[Grogu]

SeaGypsy said:


Yes, that's one of the reasons I love the pen needles, always sharp and smooth. I rarely ever feel one during the sick.

Click to expand...

Yup, love that about pen needles too. Smooth like butter entering your skin. Who knew that one puncture could dull an insulin needle that much, but it does.

 

[desinr-gal]

eidos said:


You’ll get as many answers as there are people on this forum.



From a physical chemistry perspective:

• There is no change in the state of the water during reconstitution (from solid to liquid). Therefore, there is no physical stress on the solutes.
  
• The temperature is in Kelvin in the reaction equations (I’m keeping it simple , so going from 2°C to 25°C means going from 275K to 298K. That’s less than a 10% change in temperature.
  
• For GLP-1s, there are a significant number of hydrogen bonds and hydrophobic interactions, which means that at these temperatures, the change in energy (ΔG) remains below 10%.
  
• An increase in temperature increases solubility and will break up certain hydrophobic aggregates (don’t bother looking: you can’t see them with the naked eye; if you do see them, your solution is spoiled).
  
• Adsorption onto glass walls is minimal at typical reconstitution concentrations (on the order of 1 per thousand), so it is not a factor in our situations.

In general, avoid temperatures above 25°C. Significant degradation occurs at temperatures above 30°C (deamidations, accelerated oxidation).

There’s no need to bring it up to room temperature. You can stop at around 15°C (you can easily find cases with integrated thermometers). That will be enough to increase solubility and prevent thermal shock, which some white blood cells don’t like (some people are allergic to the cold...).

So, it's fine to warm it up, and while it's not mandatory, it helps a little.

Click to expand...

It is summer time. 25C equals 77 degrees fahrenheit. Where I live it is currently about 83 degrees and will go up to 90 at some point so.. I guess it depends on where you live.

I wont be bringing mine to room temp.

 

[eidos]

desinr-gal said:


It is summer time. 25C equals 77 degrees fahrenheit. Where I live it is currently about 83 degrees and will go up to 90 at some point so.. I guess it depends on where you live.

I wont be bringing mine to room temp.

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Oh, it was even hotter in southwestern France today: 97°F/36°C. And that wasn't even the hottest day of the month!

That's why I suggested not necessarily letting the temperature rise to “room temperature.” It's better to keep an eye on what's really going on.

 

[desinr-gal]

eidos said:


Oh, it was even hotter in southwestern France today: 97°F/36°C. And that wasn't even the hottest day of the month!

That's why I suggested not necessarily letting the temperature rise to “room temperature.” It's better to keep an eye on what's really going on.

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Oh no! where in SW France are you? Im traveling there in late summer what should I prepare for temp wise?

 

[BNLFL]

SeaGypsy said:


Yes, that's one of the reasons I love the pen needles, always sharp and smooth. I rarely ever feel one during the stick.

Click to expand...

We're taking away your pen for a month and giving you syringes with 23g 1.5" needles.

 

[SeaGypsy]

BNLFL said:


We're taking away your pen for a month and giving you syringes with 23g 1.5" needles.

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But whhhyyyyy who did I piss off? I'm sorry, here's some flowers Please don't take my pens away!!!!

 

[Rezwalker]

pin straight from the fridge

enjoy the cold breeze in the glutes in the morning

Best way to start a fresh day !

 

[eidos]

desinr-gal said:


Oh no! where in SW France are you? Im traveling there in late summer what should I prepare for temp wise?

Click to expand...

You can DM me, because I don't want to publicly share too much information.

 

[Commander]

I pin right from the fridge. To me it isn’t worth the risk of possibly hurting the peptide. If you are having a problem with pinning cold, just don’t use a pen. Use an insulin syringe, draw your dose and let that syringe sit out and fet to room temperature.

If you want the convenience of using a Pen, you have to pin cold…

 

[BNLFL]

Commander said:


I pin right from the fridge. To me it isn’t worth the risk of possibly hurting the peptide. If you are having a problem with pinning cold, just don’t use a pen. Use an insulin syringe, draw your dose and let that syringe sit out and fet to room temperature.

If you want the convenience of using a Pen, you have to pin cold…

Click to expand...

You're not going to hurt it.