Peptide mass question

greenwave1015

greenwave1015

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Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
 
greenwave1015 said:

Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
Click to expand...

None of that operates in the way you're describing.
 
woundcarping said:

greenwave1015 said:

Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
Click to expand...

None of that operates in the way you're describing.
Click to expand...
Appreciate it, man.
 
Nope — filler makes up the bulk of that powder, so 10mg of powder won't equal 10mg of reta.

A possible approach: weigh out all the powder, then divide that total by 100. Since the full amount is known to hold 1000mg of reta, one-hundredth of it ought to hold 10mg of reta.
 
greenwave1015 said:

Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
Click to expand...
In theory, the underlying arithmetic checks out — provided what you're dealing with is pure Reta, and that's only true for raw API. Yet in practice, unless your scale is both extremely precise and costly, any attempt to measure out 10mg carries an error margin that throws the results off. Still, your math itself holds up. After that comes the challenge of dissolving the material, adding filler to bulk it up, breaking it apart, lyophilizing, and filling vials. Once more, I seriously doubt you're working with pure API — I'm just addressing the hypothetical. If pure bulk is what you've got, take care, man lol.

The real elephant in the room is endotoxins lol. (Presuming it is NOT pure API and what you have is ordinary lyophilized Reta) A standard .22 micron filter accomplishes precisely nothing against endotoxins, since those measure roughly .01 microns. If live bacteria or fungus are present, a filter will catch most of them... but endotoxins pass straight through, completely indifferent to whatever filter you're using.
 
unclebuff702 said:

greenwave1015 said:

Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
Click to expand...
In theory, the underlying arithmetic checks out — provided what you're dealing with is pure Reta, and that's only true for raw API. Yet in practice, unless your scale is both extremely precise and costly, any attempt to measure out 10mg carries an error margin that throws the results off. Still, your math itself holds up. After that comes the challenge of dissolving the material, adding filler to bulk it up, breaking it apart, lyophilizing, and filling vials. Once more, I seriously doubt you're working with pure API — I'm just addressing the hypothetical. If pure bulk is what you've got, take care, man lol.

The real elephant in the room is endotoxins lol. (Presuming it is NOT pure API and what you have is ordinary lyophilized Reta) A standard .22 micron filter accomplishes precisely nothing against endotoxins, since those measure roughly .01 microns. If live bacteria or fungus are present, a filter will catch most of them... but endotoxins pass straight through, completely indifferent to whatever filter you're using.
Click to expand...
Accuracy isn't the only factor here. A significant portion of that powder is made up of filler. So if you measure out 10mg of the powder, you won't be getting 10mg of reta.
 
tcpnomad said:

unclebuff702 said:

greenwave1015 said:

Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
Click to expand...
In theory, the underlying arithmetic checks out — provided what you're dealing with is pure Reta, and that's only true for raw API. Yet in practice, unless your scale is both extremely precise and costly, any attempt to measure out 10mg carries an error margin that throws the results off. Still, your math itself holds up. After that comes the challenge of dissolving the material, adding filler to bulk it up, breaking it apart, lyophilizing, and filling vials. Once more, I seriously doubt you're working with pure API — I'm just addressing the hypothetical. If pure bulk is what you've got, take care, man lol.

The real elephant in the room is endotoxins lol. (Presuming it is NOT pure API and what you have is ordinary lyophilized Reta) A standard .22 micron filter accomplishes precisely nothing against endotoxins, since those measure roughly .01 microns. If live bacteria or fungus are present, a filter will catch most of them... but endotoxins pass straight through, completely indifferent to whatever filter you're using.
Click to expand...
Accuracy isn't the only factor here. A significant portion of that powder is made up of filler. So if you measure out 10mg of the powder, you won't be getting 10mg of reta.
Click to expand...
Yeah, absolutely — though according to him, the COA reports a Peptide Assay of 96.9%. A quantification assay indicates that 96.9% of the powder in his possession is pure Reta lol (extremely improbable, but not impossible). So if what he's got is genuine, pure bulk API — no filler involved, which is why the quantification assay reads 96.9% — then yeah, the math checks out.
 
greenwave1015 said:

Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
Click to expand...
Dump it and buy a product that a trustworthy lab has genuinely analyzed, complete with a COA
 
JFC, the idea that you can just weigh the powder rests on it being homogenous — and that's not something you can count on.

unclebuff702 said:

greenwave1015 said:

Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
Click to expand...
In theory, the underlying arithmetic checks out — provided what you're dealing with is pure Reta, and that's only true for raw API. Yet in practice, unless your scale is both extremely precise and costly, any attempt to measure out 10mg carries an error margin that throws the results off. Still, your math itself holds up. After that comes the challenge of dissolving the material, adding filler to bulk it up, breaking it apart, lyophilizing, and filling vials. Once more, I seriously doubt you're working with pure API — I'm just addressing the hypothetical. If pure bulk is what you've got, take care, man lol.

The real elephant in the room is endotoxins lol. (Presuming it is NOT pure API and what you have is ordinary lyophilized Reta) A standard .22 micron filter accomplishes precisely nothing against endotoxins, since those measure roughly .01 microns. If live bacteria or fungus are present, a filter will catch most of them... but endotoxins pass straight through, completely indifferent to whatever filter you're using.
Click to expand...

Both of those points are right. A scale for measuring peptides would never be described as a “mg scale.”

It sounds like the OP doesn't plan to send a normal vial off for testing…. hundreds of dollars of testing, yet unwilling to sacrifice a vial of Reta that costs more than $30.
 
What you can see inside your vial is mostly not Reta-

A real example: an R10 vial and an R30 vial are visually identical, since 10/1000th of a gram (10mg) cannot be seen by the eye.

Once you add 2 ml of BAC, you'll have close to 2 ml of mixture—the volume barely increases.

Testing will require you to send a vial in.

This video from Jano is excellent and worth your time..
 
unclebuff702 said:

greenwave1015 said:

Suppose a friend sends you a tube with the label "1,000mg Reta powder". Apart from a "COA" claiming 99.1% purity / 96.9% peptide assay, you have no information about the contents, sterility, or whether it is significantly over- or underfilled. No vendor reviews exist. It could be flour.

Suppose you plan to mail a 10mg sample to a testing lab, using your own mg scale and your own vial.

Question is if you weighed out precisely 10mg of this powder, would approximately 9.6mg of Reta be there IF THE LABEL IS HONEST?

I could ask ChatGPT, but I'd rather hear your thoughts.

Naturally, this is entirely a hypothetical scenario.

Thanks!

PS - as for endotoxins, reconstituting with a filter probably keeps you fine (correct?)

PSS- on sterility (vial, stopper, assembly tools, BAC water, etc.), that doesn't need to be covered here
Click to expand...
In theory, the underlying arithmetic checks out — provided what you're dealing with is pure Reta, and that's only true for raw API. Yet in practice, unless your scale is both extremely precise and costly, any attempt to measure out 10mg carries an error margin that throws the results off. Still, your math itself holds up. After that comes the challenge of dissolving the material, adding filler to bulk it up, breaking it apart, lyophilizing, and filling vials. Once more, I seriously doubt you're working with pure API — I'm just addressing the hypothetical. If pure bulk is what you've got, take care, man lol.

The real elephant in the room is endotoxins lol. (Presuming it is NOT pure API and what you have is ordinary lyophilized Reta) A standard .22 micron filter accomplishes precisely nothing against endotoxins, since those measure roughly .01 microns. If live bacteria or fungus are present, a filter will catch most of them... but endotoxins pass straight through, completely indifferent to whatever filter you're using.
Click to expand...
This is solid info, and regarding what you said about endotoxins — you're spot on. So it seems an endotoxin test is unavoidable.

Also, uh, this might actually be raw API.

Thanks!
 
BikesOverCars said:

If that's what you're hinting at, please don't go buying raws and attempting any DIY
Click to expand...
I am only gathering information at this stage - nothing more. Ending up in a hospital bed isn't something I'm eager to do.

Still, pure curiosity drives me to figure out why anyone would offer raw APIs instead of the lyophilized powder, which is both "safer" and simpler to handle.
 
Picture a restaurant. Raw ingredients get purchased, kept in storage, prepared, and put together into dishes. That whole operation relies on costly gear and rigid procedures to make sure the result tastes right and won't make anyone sick. Only then does it go out as a completed plate.

We're the ones ordering the completed plate.

For me, running a restaurant is the last thing I'd sign up for. The risk is too high, the costs are too steep, the pressure is too much, and so on.
 
With 1000mg of API, you could produce 2 kits of R50. Going the API route works out less expensive since freeze drying adds cost and requires substantially more vials and glassware... on top of that, API holds up better against temperature swings and dissolves without much trouble. The people who ought to be purchasing API are those doing the finishing step (the ones running the freeze drying), and possibly compounding pharmacies that will prepare large batches of B12/BAC vials in a sterile setting with proper equipment.

Even when you own a mg scale, using one is tough. To actually weigh this, an analytical balance is what you need!
 
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