Picked up a NAD+ kit, but I'm clueless about how to actually use it. Whether it's buffered — or what that even means — I have no idea. After watching a few videos, I'm leaning toward reconstituting with 5ml, though dosing and timing remain unclear, and dr.google gives me plenty of contradictory answers. Any help would be appreciated.
NAD+ usage guidance needed
- Thread starter ambot88
- Start date
Fostradamus
Explorer
I used this as a guide, and I've worked my way up to 100mg taken every other day.
Pokemon999
Explorer
Right now I'm on 40mg daily. When you hit 100mg, did you feel any change?
catsincali
Explorer
thanks for posting this!!!
leeann82
Explorer
Bookmarking this. NAD is going on my next order.
SolidChick
Explorer
At the moment, my daily intake is roughly 50-75 mg.
SolidChick
Explorer
I began at 25mg daily, then bumped it up to 50mg based on the dosing chart. Since I noticed no effects, I went up to 75mg.
Still nothing. I plan to add motsc before long.
Loveandpeaceandjoy
Explorer
RS started out using this protocol. These days it's 80mg taken 3x per week. It's given a big lift to weight loss. Planning to bring in 5AM before long. Gave Mots a go but had an allergic reaction.
bonelifts
Explorer
My wife and I are currently taking 40mg with a schedule of 4 days on and 1 day off, which lines up with our training schedule. We have it as part of our preworkout combination: Mots-C, 5-Amino, and NAD.
Calm Logic
Explorer
Among these, MOTS-c appears to be the biggest offender when it comes to setting off an adverse response ("your immune system trying to...quarantine the "danger molecules" you just introduced").
I ran NAD+ as part of the complete MITO stack: ss-31/MOTSc/NAD+
My dose was 100 mg on MWF
for the term: see below
I also used TMG to replenish Methyl
Practical Recommendations for NAD+ & TMG Supplementation#-practical-recommendations-for-nad-tmg-supplementation
- Timing and Dosage
Given what is currently known, TMG works as a long-acting methyl donor, so it may be taken at any time of the day. Nothing indicates that TMG has to be paired with NAD+ at the same moment. A standard amount is 1,000mg of TMG daily, which ought to cover methylation needs with room to spare. That said, if taking TMG every day feels like a burden, using it just on the days when you apply your NAD+ patch is a workable alternative.
- Daily vs. Occasional Supplementation
Some patients choose daily TMG, while others use it only alongside their NAD+ patches. Neither approach is wrong, and how each person responds may differ according to their own methylation capacity.
- Methylation Testing
At present, no precise tests exist that measure methylation efficiency or NAD+ metabolism well enough to guide individualized TMG dosing. Even so, genetic testing for MTHFR mutations and measuring homocysteine levels can offer clues about your methylation capacity.
At Sellers website, we’ll keep watching the newest research and offer revised guidance as more becomes clear about how NAD+ and methyl donors such as TMG interact.
Using Trimethylglycine (TMG) together with NAD+ precursors (such as NMN or NR) restores methyl groups that get used up while NAD+ levels are being raised. Pairing them this way helps maintain optimal methylation, which matters for DNA repair, detoxification, and keeping homocysteine from running high, and they are often taken together at a 1:1 ratio.
Key Aspects of Combining TMG with NAD+ Boosters:
- Why Combine Them: NAD+ boosters support energy production yet may use up the body’s supply of methyl groups. TMG acts as a "methyl donor," returning those groups so methylation pathways stay open.
- Optimal Ratio & Dosage: Experts, including Dr. David Sinclair, frequently suggest a 1:1 ratio of TMG to NMN/NR, usually about
When to Take: TMG may be taken in the morning, often alongside food to improve absorption and avoid stomach upset.
of TMG daily.
- Synergy: NMN/NR raises NAD+ directly, while TMG keeps the metabolic process from causing methyl groups to be depleted over the long term, supporting cellular health overall.
- Benefits: Possible benefits include lasting energy, better cardiovascular health, and improved methylation, with notable improvements frequently reported after 3-6 months.
Fostradamus
Explorer
I wish I could give a straight yes, but I have to add that NAD isn't the only thing I'm using. My energy has gone up, though reta is in the mix too, so that boost might come just from losing weight, or from NAD and reta working together. Before I switched to reta, I was on Wegovy and no NAD, and compared with then, my energy is far higher now. Interpret that however you like.
Neptide
Explorer
Studies indicate increased energy levels when combining NAD+ with Tirz. Following a period of fatigue while on Tirz, NAD+ proved to be a significant improvement.
Foggy-Hollow
Explorer
For my RS on tirz, electrolytes made a huge difference. I went from sleeping through most of Saturday to actually getting things done. (Shot on Friday evening)
My NAD dose has stayed the same — 25-50mg, 3 times per week, occasionally less, and I'm not on tirz right now. The Adhedonia didn't go away, but my energy floor came up, so I've stuck with it.
If you're under 40, your NAD+ recycling and production systems might not be as run down. In that situation, you might not get the same boost that others report.
Only way to find out is to try it.
Even buffered, it's spicy. I recon 2.5 mL into 250 mg vials, then push the plunger slowly. Some days it's fine, other days I need to stop 2-3-4 times.
1u= 1mg, which keeps the math simple.
Tirz holds up very well, but my NAD+ clearly stops working as well after roughly 4 weeks. That's why I doubt I'll ever buy 500mg vials.
My NAD dose has stayed the same — 25-50mg, 3 times per week, occasionally less, and I'm not on tirz right now. The Adhedonia didn't go away, but my energy floor came up, so I've stuck with it.
If you're under 40, your NAD+ recycling and production systems might not be as run down. In that situation, you might not get the same boost that others report.
Only way to find out is to try it.
Even buffered, it's spicy. I recon 2.5 mL into 250 mg vials, then push the plunger slowly. Some days it's fine, other days I need to stop 2-3-4 times.
1u= 1mg, which keeps the math simple.
Tirz holds up very well, but my NAD+ clearly stops working as well after roughly 4 weeks. That's why I doubt I'll ever buy 500mg vials.
I don't think your question about buffering ever got a direct answer. Some time ago, NAD+ was being mixed to such an acidic state that it amounted to injecting acid, leading to severe reactions at the injection site, or possibly worse. The idea behind "buffering" was to achieve a more stable and usable pH.
From what I've seen, even products labeled as buffered tended to remain acidic. There are lengthy discussions about the ideal pH. Here's what Gemini AI has to say:
"Although NAD+ is most stable at lower pH levels, the human body's physiological pH is approximately 7.4.
Sadly, I've ended up testing and tweaking every batch of NAD+ I've gotten, regardless of any buffered claim. Get some pH strips from Amazon—they're inexpensive. Also pick up injectable Sodium Bicarbonate (definitely not baking soda). After that, it's purely trial and error. People approach it differently. If my goal is 5ml in a 500mg vial, I'll first put in 3ml of BAC, then gradually introduce sodium bicarbonate while checking pH until it lands in the target range. Once I know how much SB went in, I top it off with the remaining BAC water and check pH one last time. I can usually hit the range, though it's not an exact science. Even so, it beats injecting acid.
From what I've seen, even products labeled as buffered tended to remain acidic. There are lengthy discussions about the ideal pH. Here's what Gemini AI has to say:
"Although NAD+ is most stable at lower pH levels, the human body's physiological pH is approximately 7.4.
- Irritation Threshold: Injections with a pH below 3.0 or above 9.0 typically cause significant pain, burning, and tissue irritation at the site of administration.
- Injection Site Comfort: A pH of 5.0 to 6.0 is generally tolerable for subcutaneous or intramuscular injections, providing a balance that preserves the potency of the NAD+ for several weeks (when refrigerated) without causing severe discomfort."
Sadly, I've ended up testing and tweaking every batch of NAD+ I've gotten, regardless of any buffered claim. Get some pH strips from Amazon—they're inexpensive. Also pick up injectable Sodium Bicarbonate (definitely not baking soda). After that, it's purely trial and error. People approach it differently. If my goal is 5ml in a 500mg vial, I'll first put in 3ml of BAC, then gradually introduce sodium bicarbonate while checking pH until it lands in the target range. Once I know how much SB went in, I top it off with the remaining BAC water and check pH one last time. I can usually hit the range, though it's not an exact science. Even so, it beats injecting acid.
Calm Logic
Explorer
It only just clicked for me that NAD+ offers skin and collagen protection, though its role leans more toward defense than the proactive action of GHK-Cu.
When used topically, NAD+ comes in serum form, and so does NMN, the precursor. However, for these serums to actually work, they need to be applied with a tool such as a dermapen or dermaroller (this is covered in the attached information).
If you're committed to the topical route, PWGrove is currently running a GB for an NMN serum ($85/kit + fees):
When used topically, NAD+ comes in serum form, and so does NMN, the precursor. However, for these serums to actually work, they need to be applied with a tool such as a dermapen or dermaroller (this is covered in the attached information).
If you're committed to the topical route, PWGrove is currently running a GB for an NMN serum ($85/kit + fees):