KPV and filtering

GrandmaJ

GrandmaJ

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I'm finally getting to the compounded triz I've had stockpiled, so reconstituting and filtering is new for me. Reconstitution went smoothly, but pulling the solution from its original vial into the 3ml syringe didn't go as planned—only about 2 of the 3ml came up, mixed with a large amount of air, which filled the syringe and blocked me from getting the remainder. Pushing it back and starting over produced the same result. There's clearly some basic technique I'm overlooking. On the Youtube clips it looks completely effortless!
 
GrandmaJ said:

I'm finally getting to the compounded triz I've had stockpiled, so reconstituting and filtering is new for me. Reconstitution went smoothly, but pulling the solution from its original vial into the 3ml syringe didn't go as planned—only about 2 of the 3ml came up, mixed with a large amount of air, which filled the syringe and blocked me from getting the remainder. Pushing it back and starting over produced the same result. There's clearly some basic technique I'm overlooking. On the Youtube clips it looks completely effortless!
Click to expand...
A 1.5 inch long needle does the job nicely. Begin by adding air until no vacuum remains.

When the vial holds a lot of peptide solution, you might need to alternate: tilt the bottle so air leaves the syringe, then draw additional liquid, repeating as needed.

Another option is to reconstitute with a smaller amount of bac, then use the remaining required volume to flush your filter into the new vial. Should you skip filtering (though I strongly suggest you filter), the remainder can go straight into the new vial to achieve the correct dilution.
 
Nearly every one of these issues comes down to a difference in pressure.

Add a vent and the problem goes away.

You don't need any fancy vent filter needle. Just grab the least expensive syringe you own, take out the plunger, and insert it.
 
yrrdead said:

Nearly every one of these issues comes down to a difference in pressure.

Add a vent and the problem goes away.

You don't need any fancy vent filter needle. Just grab the least expensive syringe you own, take out the plunger, and insert it.
Click to expand...
That's particularly the case when you're pulling liquid out of the vial you reconstituted in. But I ran into the very same issue despite having a vent — and foolishly I'd put a filter on it. The air didn't move the way I expected, so I resorted to tilting, and ended up with peptide inside the filter. What a disaster. And pointless too, because I was planning to filter it afterward regardless. So frustrating.

If you're filtering into a cartridge or vial that you'll be drawing from for weeks or more, it might be worth adding a vent filter.
 
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