Filter Waste

[SailorofSeas]

How much waste from filters should I expect?

My first attempt with a .22 micron PES filter just now, and I'm pretty sure the entire 1.5 ml of product disappeared.

Is filtering just a trick to get me to waste my peps?

 

[BNLFL]

A few YouTube clips cover this topic. The typical approach: draw roughly 15 units of BAC into its own syringe, then inject that through to rinse out the filtered vial.

View: https://www.youtube.com/watch?v=0EHHXTdlYCw

 

[HereKittyKitty]

View: https://youtu.be/9YBj26vpHs0?si=TrEbLbu82MYzCvwR

 

[agussie]

Using a 4mm filter, I get the impression I might be losing around 3-5 units or so

 

[HereKittyKitty]

agussie said:

Using a 4mm filter, I get the impression I might be losing around 3-5 units or so

Did you have any problems with the filter bursting? From what I've read, pushing over 1ml through a 4mm filter will probably blow it out unless you take it extremely slowly.

 

[BNLFL]

HereKittyKitty said:

agussie said:

Using a 4mm filter, I get the impression I might be losing around 3-5 units or so

Did you have any problems with the filter bursting? From what I've read, pushing over 1ml through a 4mm filter will probably blow it out unless you take it extremely slowly.

I've come across the same thing too.

 

[Turbo-Farmer]

HereKittyKitty said:

agussie said:

Using a 4mm filter, I get the impression I might be losing around 3-5 units or so

Did you have any problems with the filter bursting? From what I've read, pushing over 1ml through a 4mm filter will probably blow it out unless you take it extremely slowly.

In my view, what matters more than the volume is how much differential pressure you're putting on it. When you push down on the plunger to filter, you're applying a set amount of force. With a bigger filter, that force gets spread out over a wider area. So with a 4 mm filter, remember that it's simple to push too hard and rip the membrane.

 

[Chili777]

For anything 3 mL or less, I go with a 4 mm filter; once it's 3+ mL, I switch to 13 mm. Because of the hold back volume, I air purge the 4 mm and BAC purge the 13 mm.

Depending on who made it, hold up volume for 4 mm ought to be 2-4 IU, while 13 mm sits at roughly 7-10 IU.

 

[HereKittyKitty]

Turbo-Farmer said:

HereKittyKitty said:

agussie said:

Using a 4mm filter, I get the impression I might be losing around 3-5 units or so

Did you have any problems with the filter bursting? From what I've read, pushing over 1ml through a 4mm filter will probably blow it out unless you take it extremely slowly.

In my view, what matters more than the volume is how much differential pressure you're putting on it. When you push down on the plunger to filter, you're applying a set amount of force. With a bigger filter, that force gets spread out over a wider area. So with a 4 mm filter, remember that it's simple to push too hard and rip the membrane.

That's exactly why I told you to take your time with a 4mm filter. A 4mm is meant for amounts under 1ml, while a 13mm handles 1-10ml.

 

[Chili777]

HereKittyKitty said:

Turbo-Farmer said:

HereKittyKitty said:

agussie said:

Using a 4mm filter, I get the impression I might be losing around 3-5 units or so

Did you have any problems with the filter bursting? From what I've read, pushing over 1ml through a 4mm filter will probably blow it out unless you take it extremely slowly.

In my view, what matters more than the volume is how much differential pressure you're putting on it. When you push down on the plunger to filter, you're applying a set amount of force. With a bigger filter, that force gets spread out over a wider area. So with a 4 mm filter, remember that it's simple to push too hard and rip the membrane.

That's exactly why I told you to take your time with a 4mm filter. A 4mm is meant for amounts under 1ml, while a 13mm handles 1-10ml.

For volumes below 3 mL, a 13 mm filter just squanders peptide. Sure, opinions differ, but my goal is to get as much peptide as possible into the vial. Applying steady pressure of 1 or 2 lb does the job well.

 

[Turbo-Farmer]

HereKittyKitty said:

Turbo-Farmer said:

HereKittyKitty said:

agussie said:

Using a 4mm filter, I get the impression I might be losing around 3-5 units or so

Did you have any problems with the filter bursting? From what I've read, pushing over 1ml through a 4mm filter will probably blow it out unless you take it extremely slowly.

In my view, what matters more than the volume is how much differential pressure you're putting on it. When you push down on the plunger to filter, you're applying a set amount of force. With a bigger filter, that force gets spread out over a wider area. So with a 4 mm filter, remember that it's simple to push too hard and rip the membrane.

That's exactly why I told you to take your time with a 4mm filter. A 4mm is meant for amounts under 1ml, while a 13mm handles 1-10ml.

In my case, what matters more is how many mg of peptides get trapped by the filter. The BAC isn't what causes the clogging—it's the contaminants you're attempting to eliminate.

 

[Chili777]

Turbo-Farmer said:

HereKittyKitty said:

Turbo-Farmer said:

HereKittyKitty said:

agussie said:

Using a 4mm filter, I get the impression I might be losing around 3-5 units or so

Did you have any problems with the filter bursting? From what I've read, pushing over 1ml through a 4mm filter will probably blow it out unless you take it extremely slowly.

In my view, what matters more than the volume is how much differential pressure you're putting on it. When you push down on the plunger to filter, you're applying a set amount of force. With a bigger filter, that force gets spread out over a wider area. So with a 4 mm filter, remember that it's simple to push too hard and rip the membrane.

That's exactly why I told you to take your time with a 4mm filter. A 4mm is meant for amounts under 1ml, while a 13mm handles 1-10ml.

In my case, what matters more is how many mg of peptides get trapped by the filter. The BAC isn't what causes the clogging—it's the contaminants you're attempting to eliminate.

My approach is based on how much liquid I use to reconstitute. Whether it's 30 mg of Semax or 10 mg, they appear identical. One is mixed with 500 mcg of BAC, the other with 1 mL of BAC, and regardless, I use the same 4 mm filter.