DIY Tesamorelin/Ipamorelin Blend: The Science and the Math

[TheEngineer]

MIXING IPAMORELIN INTO A TESAMORELIN VIAL FOR AN EASIER DOSE WITH FEWER PINS.

HOW TO LAND 1MG TESA + 200mcg IPA EVERY DOSE​

1. Neither peptide settles out — this is a real solution, not a suspension

• Tesamorelin and Ipamorelin both come as acetate salts of peptides. In bacteriostatic water they are highly water-soluble.
  
• Once dissolved, the peptide molecules sit as individual molecules (or tiny solvated clusters) spread through the water.
  
• That makes it a true molecular solution, not a suspension of particles that could settle or clump.

2. Brownian motion + diffusion = rapid, complete mixing

• In any liquid at room temperature, molecules are constantly in random motion from thermal energy (this is called Brownian motion).
  
• That random movement spreads molecules out of high-concentration areas and into low-concentration ones until everything matches across the vial.
  
• The process follows Fick’s laws of diffusion. For peptides of this size in water, the diffusion coefficient is large enough that complete uniformity arrives within seconds to a few minutes of gentle swirling in a small 2–4 ml vial.
  
• Swirling also sets up convection currents, which speed the process up even more.

At that point the solution is homogeneous (uniform at the molecular level). There is no longer any “more Ipa in one part of the vial” or “more Tesa in another part.”

3. Nothing exists that would let the peptides separate again

• Both peptides are hydrophilic (water-loving) and behave similarly in bac water.
  
• No chemical reaction, precipitation, crystallization, or hydrophobic effect would push one peptide to clump or split away from the other.
  
• At this scale gravity does nothing meaningful to dissolved molecules (they do not “settle” like sand in water).
  
• Entropy (the natural drift toward disorder) in fact favors the mixed state.

So once mixed, the solution stays homogeneous indefinitely (as long as the peptides don’t degrade from heat, light, or time).

4. Which means every accurately measured volume holds the exact proportion

• Concentration = peptide mass ÷ solution volume.
  
• Since the solution is homogeneous, any subsample (e.g. 0.2 ml) carries the exact same concentration as the whole vial.
  
• That is how pharmacies build compounded multi-peptide vials that deliver consistent doses from the first draw to the last.

In practice this is the same principle behind insulin pens, multi-vitamin IV bags, and thousands of daily peptide users mixing compounds reliably. The science of homogeneity holds up — the solution will be perfectly even.

The Math, Step by Step

1. Reconstituting the Tesamorelin

10 mg of Tesamorelin + 1.6 ml of bac water

→ So the concentration = 10 mg ÷ 1.6 ml, which gives 6.25 mg/ml

2. Reconstituting the Ipamorelin

10 mg of Ipamorelin + 2 ml of bac water

→ Which gives a concentration of 5 mg/ml

3. Transferring it

Into the Tesamorelin vial, add 0.4 ml of the Ipamorelin solution.

Ipamorelin added = 0.4 ml × 5 mg/ml, which is 2 mg

4. The finished mixed vial

o Tesamorelin total = 10 mg

o Ipamorelin total = 2 mg

o Overall volume = 1.6 ml + 0.4 ml = 2.0 ml

What that leaves you with:

o Tesamorelin comes to 10 mg ÷ 2.0 ml = 5 mg/ml

o Ipamorelin comes to 2 mg ÷ 2.0 ml = 1 mg/ml

5. Drawing a shot

Pull 0.2 ml (20 units on a U-100 insulin syringe) out of the mixed vial.

What lands in the syringe:

o From Tesamorelin: 0.2 ml × 5 mg/ml = 1 mg

o From Ipamorelin: 0.2 ml × 1 mg/ml = 0.2 mg = 200 mcg

A perfect 1:1 ratio, every single time.



​

 

[TheEngineer]

CALCULATIONS FOR 2MG TESA + 200mcg IPA PER INJECTION

Step-by-Step Arithmetic

1. Preparing Ipamorelin (no change)

10 mg Ipamorelin + 2 ml bac water

→ Strength = 5 mg/ml

2. Desired final strengths in the combined vial

o To get 2 mg Tesamorelin from a 0.4 ml draw → Tesamorelin strength must be 5 mg/ml

o To get 0.2 mg Ipamorelin from a 0.4 ml draw → Ipamorelin strength must be 0.5 mg/ml

3. Total volume of the mixed vial

You have 10 mg Tesamorelin total.

At 5 mg/ml, the total volume must be:

V = 10 mg ÷ 5 mg/ml = 2.0 ml

4. How much Ipamorelin to include

For 0.5 mg/ml in a 2.0 ml vial:

Total Ipamorelin required = 0.5 mg/ml × 2.0 ml = 1 mg

5. Volume of Ipamorelin to withdraw

Since Ipamorelin is 5 mg/ml:

Volume to transfer = 1 mg ÷ 5 mg/ml = 0.2 ml

6. How much bac water for Tesamorelin

The final volume needs to be 2.0 ml, and you're adding 0.2 ml of Ipamorelin.

So reconstitute the 10 mg Tesamorelin vial with:

1.8 ml bacteriostatic water

Quick Reference

Step​

Action​

Result​

1Mix 10 mg Tesamorelin with 1.8 ml bac water5.556 mg/ml Tesa2Mix 10 mg Ipamorelin with 2 ml bac water5 mg/ml Ipa3Inject 0.2 ml of the Ipamorelin solution into the Tesamorelin vialFinal volume = 2.0 ml4Swirl gently until dissolvedUniform solution

Injection Draw

Pull 0.4 ml (40 units) from the combined vial.

This gives you precisely:

• Tesamorelin = 0.4 ml × 5 mg/ml = 2 mg
  
• Ipamorelin = 0.4 ml × 0.5 mg/ml = 0.2 mg = 200 mcg

Consistent every time.

 

[UpDownLeftRightAS*]

That's excellent. Ipa's effectiveness does shift as you get older, so would you think about raising that 200 mcg for the higher age brackets?

 

[JoonyO]

TheEngineer said:

CALCULATIONS FOR 2MG TESA + 200mcg IPA PER INJECTION

Step-by-Step Arithmetic

1. Preparing Ipamorelin (no change)

10 mg Ipamorelin + 2 ml bac water

→ Strength = 5 mg/ml

2. Desired final strengths in the combined vial

o To get 2 mg Tesamorelin from a 0.4 ml draw → Tesamorelin strength must be 5 mg/ml

o To get 0.2 mg Ipamorelin from a 0.4 ml draw → Ipamorelin strength must be 0.5 mg/ml

3. Total volume of the mixed vial

You have 10 mg Tesamorelin total.

At 5 mg/ml, the total volume must be:

V = 10 mg ÷ 5 mg/ml = 2.0 ml

4. How much Ipamorelin to include

For 0.5 mg/ml in a 2.0 ml vial:

Total Ipamorelin required = 0.5 mg/ml × 2.0 ml = 1 mg

5. Volume of Ipamorelin to withdraw

Since Ipamorelin is 5 mg/ml:

Volume to transfer = 1 mg ÷ 5 mg/ml = 0.2 ml

6. How much bac water for Tesamorelin

The final volume needs to be 2.0 ml, and you're adding 0.2 ml of Ipamorelin.

So reconstitute the 10 mg Tesamorelin vial with:

1.8 ml bacteriostatic water

Quick Reference

Step​

Action​

Result​

1Mix 10 mg Tesamorelin with 1.8 ml bac water5.556 mg/ml Tesa2Mix 10 mg Ipamorelin with 2 ml bac water5 mg/ml Ipa3Inject 0.2 ml of the Ipamorelin solution into the Tesamorelin vialFinal volume = 2.0 ml4Swirl gently until dissolvedUniform solution

Injection Draw

Pull 0.4 ml (40 units) from the combined vial.

This gives you precisely:

• Tesamorelin = 0.4 ml × 5 mg/ml = 2 mg
  
• Ipamorelin = 0.4 ml × 0.5 mg/ml = 0.2 mg = 200 mcg

Consistent every time.

I have a question.

Lately there's been quite a bit of discussion about keeping Tesa at room temperature (and finishing the reconstituted vial within 7 days), which makes me curious how that applies to storing the blend.

Any input?

 

[Calm Logic]

JoonyO said:

TheEngineer said:

CALCULATIONS FOR 2MG TESA + 200mcg IPA PER INJECTION

Step-by-Step Arithmetic

1. Preparing Ipamorelin (no change)

10 mg Ipamorelin + 2 ml bac water

→ Strength = 5 mg/ml

2. Desired final strengths in the combined vial

o To get 2 mg Tesamorelin from a 0.4 ml draw → Tesamorelin strength must be 5 mg/ml

o To get 0.2 mg Ipamorelin from a 0.4 ml draw → Ipamorelin strength must be 0.5 mg/ml

3. Total volume of the mixed vial

You have 10 mg Tesamorelin total.

At 5 mg/ml, the total volume must be:

V = 10 mg ÷ 5 mg/ml = 2.0 ml

4. How much Ipamorelin to include

For 0.5 mg/ml in a 2.0 ml vial:

Total Ipamorelin required = 0.5 mg/ml × 2.0 ml = 1 mg

5. Volume of Ipamorelin to withdraw

Since Ipamorelin is 5 mg/ml:

Volume to transfer = 1 mg ÷ 5 mg/ml = 0.2 ml

6. How much bac water for Tesamorelin

The final volume needs to be 2.0 ml, and you're adding 0.2 ml of Ipamorelin.

So reconstitute the 10 mg Tesamorelin vial with:

1.8 ml bacteriostatic water

Quick Reference

Step​

Action​

Result​

1Mix 10 mg Tesamorelin with 1.8 ml bac water5.556 mg/ml Tesa2Mix 10 mg Ipamorelin with 2 ml bac water5 mg/ml Ipa3Inject 0.2 ml of the Ipamorelin solution into the Tesamorelin vialFinal volume = 2.0 ml4Swirl gently until dissolvedUniform solution

Injection Draw

Pull 0.4 ml (40 units) from the combined vial.

This gives you precisely:

• Tesamorelin = 0.4 ml × 5 mg/ml = 2 mg
  
• Ipamorelin = 0.4 ml × 0.5 mg/ml = 0.2 mg = 200 mcg

Consistent every time.

I have a question.

Lately there's been quite a bit of discussion about keeping Tesa at room temperature (and finishing the reconstituted vial within 7 days), which makes me curious how that applies to storing the blend.

Any input?

That bro science nonsense was already covered in a previous discussion:


/community-link/thread/11074/


The prescription formulation of tesa is built to a different spec. Once we reconstitute, sterility becomes our concern because the Chinese powder contains no preservatives.

 

[JoonyO]

Thanks for the link to the discussion.